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Growth and biopigment accumulation of cyanobacterium Spirulina platensis at different light intensities and temperature BJM
Kumar,Manoj; Kulshreshtha,Jyoti; Singh,Gajendra Pal.
In order to find out optimum culture condition for algal growth, the effect of light irradiance and temperature on growth rate, biomass composition and pigment production of Spirulina platensis were studied in axenic batch cultures. Growth kinetics of cultures showed a wide range of temperature tolerance from 20 ºC to 40 ºC. Maximum growth rate, cell production with maximum accumulation of chlorophyll and phycobilliproteins were found at temperature 35 ºC and 2,000 lux light intensity. But with further increase in temperature and light intensity, reduction in growth rate was observed. Carotenoid content was found maximum at 3,500 lux. Improvement in the carotenoid content with increase in light intensity is an adaptive mechanism of cyanobacterium...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Spirulina platensis; Growth kinetics; Chlorophyll a; Carotenoid; Phycobiliproteins.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300034
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Role of Light and Dark Cycle and Different Temperatures in the Regulation of Growth and Protein Expression in Oscillatoria agardhii Strain BABT
Dautania,Gajendra Kumar; Singh,Gajendra Pal.
The cyanobacterium Oscillatoria agardhii was isolated from the fresh water Mawatha lake, Jaipur and was grown in Zarrouk's medium at 25 ± 2°C, illuminated with white fluorescent light at the intensity of 2 500 lux with 12:12 h light and dark photoperiod. The effects of photoperiod and temperature on the growth and protein expression by Oscillatoria agardhii were studied under different controlled culture conditions (ALR, ALC, CLR, CLC, and NDL), measuring optical density, cell count and dry weight. Protein content was measured quantitatively by Bradford assay and qualitatively by SDS-PAGE. The densitometric analysis was also carried out for the measurement of the expression level of different proteins/peptides under different culture conditions. Maximum...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bradford assay; Densitometer; Oscillatoria agardhii; Photoperiod; SDS-PAGE.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000600933
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